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DNA Concentration Calculator

DNA concentration from absorbance at 260 nm.

Compute DNA concentration in ng/μL from a spectrophotometer absorbance reading at 260 nm and the cuvette dilution factor.

Daniel Oh By Daniel Oh
Reviewed by Dr. Helena Ortiz
Published Last reviewed 1 min read

Inputs

Results

Enter values and click Calculate to see results.
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How to use this calculator

  1. Fill in the inputs above using the units you already have.
  2. Values update automatically as you type — no submit button needed.
  3. Hover any result row for the underlying formula and intermediate values.

Formula

[DNA] (ng/μL) = A₂₆₀ · 50 · dilution factor for double-stranded DNA.

In depth

Compute DNA concentration in ng/μL from a spectrophotometer absorbance reading at 260 nm and the cuvette dilution factor.