basic

Lineweaver-Burk Plot Calculator

Reciprocal transform of Michaelis-Menten data.

Compute the Lineweaver-Burk reciprocals 1/v and 1/[S] from a single substrate concentration and reaction velocity, plus the slope and intercept of the linearised Michaelis-Menten plot.

Priya Venkatesan By Priya Venkatesan
Reviewed by Dr. Helena Ortiz
Published Last reviewed 1 min read

Inputs

mM
µmol/min
mM
µmol/min

Results

Enter values and click Calculate to see results.
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How to use this calculator

  1. Fill in the inputs above using the units you already have.
  2. Values update automatically as you type — no submit button needed.
  3. Hover any result row for the underlying formula and intermediate values.

Formula

1/v = (Km / Vmax) · (1/[S]) + 1/Vmax

In depth

Compute the Lineweaver-Burk reciprocals 1/v and 1/[S] from a single substrate concentration and reaction velocity, plus the slope and intercept of the linearised Michaelis-Menten plot.